Digested the srlD and pET28a with BamHI and HindIII
Extracted the digestion and performed a ligation which ran overnight at 4C
Another attempt to PCR srl Operon success! 7 of 8 reactions correct band placement on gel
Digestion and Ligations performed. Success! Team Fungus:
Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.
Ran gradient PCR with diluted cDNA.
Ran nested PCR with diluted cDNA.
Ran nested touchdown PCR with diluted cDNA.
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