Team:Rice University/Notebook/21 May 2008

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Wednesday 21 May

  • Taylor Stevenson
    • Transformation From New Registry (Paper Punches)-Electroporation of [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 BBa_I13602] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into TetR electrocompetent cells.
    1. Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min
    2. Electropulse parameters: 1.8 kV & 5.4 ms
    3. plated 20uL of 1/1, 1/100, 1/10,000 dilutions and 100uL of 1/1 on LB/Amp plates. Plates were cultured O/N.
    • Result-no colonies were observed at any dilution






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