Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)

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design

Sender

  • [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI(BBa_K084007)]

Tet-lasi chiba.gif

  • [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI(BBa_K084008)]

Tet-rhli chiba.gif

  • [http://partsregistry.org/Part:BBa_S03623 ptet+rbs+LuxI(LVA)(BBa_S03623)]

Chiba BBa S03623.gif

Receiver

  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Reporter 9002 chiba.gif

Method

  1. Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37°C 12h
  3. Mixed them.
  4. Incubated at 37°C.
  5. Measured intensity of green fluorescence at regular time intervals.

more details...

Results

Reaction temparature:37°C

  • センダーの培養液：500μL、レシーバの培養液：500μL
Fig.　　
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.

Reaction temparature:30°C

  • センダーの培養液：500μL、レシーバの培養液：500μL
Fig.　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.


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