Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)
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design
Sender
- [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI(BBa_K084007)]
- [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI(BBa_K084008)]
- [http://partsregistry.org/Part:BBa_S03623 ptet+rbs+LuxI(LVA)(BBa_S03623)]
Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
Method
- Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 37°C.
- Measured intensity of green fluorescence at regular time intervals.
Results
Reaction temparature:37°C
- センダーの培養液:500μL、レシーバの培養液:500μL
Reaction temparature:30°C
- センダーの培養液:500μL、レシーバの培養液:500μL
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