Team:PennState/diauxie/progress

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Diauxie Elimination

Introduction
The System
Strategies
Progress
Conclusions
Parts
References

NHR Biosensors

NHR Introduction
Phthalate Biosensor
BPA Biosensor
 Progress & Results

We have completed our first set of cloning phases and are conducting our first round of testing and modification. Each test construct (promoter + GFP) has been cloned into pSB1A2 and transformed into DH5α, W3110 ∆xylB-G, and W3110 ∆xylB-R. Preliminary induction studies were run to find the optimal induction time and to see in what range OD and fluorescence were linearly related.

Test Construct
[Test Consturct]
Promoter pSB1A2 DH5α W3110 ΔxylB-G W3110 ΔxylB-R
PN
P1
P3
P5

Test were then run to compare the level of induction between mixes of xylose, glucose and xylose, and glucose. The goal of this project is to obtain a noticeably higher level of induction when induced with xylose and glucose compared to the wildtype construct.

DH5a fluoresence

W3110 fluoresence

PN induction time

P1 induction time

P3 induction time

PN linear range

P1 linear range

P3 linear range

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