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Contents 1 Parts Submitted to the Registry 1.1 GFP 1.2 p1025 1.3 L.bulgaricus signal 1.4 FLAG tag 1.5 HA tag 1.6 His tag 1.7 Tev Cleavage Site 1.8 L. bulgaricus Native Promoter 1.9 pLEM415 plasmid with Biobrick inserts 1.10 pJK650 plasmid

Parts Submitted to the Registry

Please check back later for BioBrick numbers.

GFP

Although there are multiple GFP parts in the registry already, this part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

• BioBrick Number:

p1025

This sequence codes for a short peptide, found to competitively inhibit binding of S.mutans to the tooth surface ([http://www.ncbi.nlm.nih.gov/pubmed/9920267?dopt=Abstract|Kelly CG et al.; Nature Biotechnol. 1999]). S.mutans takes in sugars and secretes lactic acid, causing dental cavities, so introduction of this peptide into the mouth prevents colonies. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

• BioBrick Number:

L.bulgaricus signal

This is a short signal sequence recognized as a secretion tag by Lactobacillus strains. It was isolated from the 5' end of the prtB gene in Lactobacillus bulgaricus, which codes for a lactose digesting protein that is bound to the surface ([http://www.ncbi.nlm.nih.gov/pubmed/12788739?dopt=Abstract| Germond JE et al.; Appl Environ Microbiol. 2003]). This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

• BioBrick Number:

FLAG tag

This is the well-known FLAG tag for use in antibody binding assays. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

• BioBrick Number:

HA tag

This is the well-known HA tag for use in antibody binding assays. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

• BioBrick Number:

His tag

This codes for six Histidine residues to allow for His column protein purification. It will work best if put at either the N or C terminus of your protein construct. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

• BioBrick Number:

Tev Cleavage Site

This codes for the seven amino acid sequences that is recognized by the Tobacco Etch Virus protease. The amino acid sequenced used is:

• Glu-Asn-Leu-Tyr-Phe-Gln-Gly.

The TEV protease cuts between Gln and Gly. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

• BioBrick Number:

L. bulgaricus Native Promoter

This is the native LacS Promoter of the bacteria Lactobacillus delbruckii subsp. bulgaricus. It is a LacS promoter taken from genomic DNA. It should include a ribosome binding site also, so no additional RBS is needed. This promoter should be used in experiments that aim to alter the gene function of L. bulgaricus. It is a LacS promoter so should be able to be regulated.

• Biobrick Number: [http://partsregistry.org/Part:BBa_K128006 BBa_K128006]

pLEM415 plasmid with Biobrick inserts

This is the pLEM415 plasmid, an E.coli shuttle vector and is able to transform Lactobacillus delbruckii subsp. bulgaricus and Lactobacillus delbruckii subsp. lactis.

• Biobrick Number: [http://partsregistry.org/Part:BBa_K128007 BBa_K128007]

pJK650 plasmid

This is the pJK650 plasmid, which has the ability to transform Lactobacillus delbruckii subsp. bulgaricus and Lactobacillus delbruckii subsp. lactis.

• Biobrick Number: [http://partsregistry.org/Part:BBa_K128008 BBa_K128008]

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

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