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Team:EPF-Lausanne/Modeling
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Double input model
Here we describe a semi-quantitative model of the Lux band-pass detector and the Rhl high-pass detector. The model describes each proteins and sending molecules involved in the system.
States
ODEs
Parameters
Simulation
Bistable switch model
States
ODEs
Parameters
Simulation
[GFP (left) and RFP (middle) expression for concentration of AHL (Lux or Rhl) pulses. On the right, figure shows the ratio of GFP/RFP.
[Image:bistabl_sim.jpg]]
The following figures show the dynamics of the different states involved in the system for different AHL pulses. On the left, the system is locked into the green state after a pulse of 1.6 uM of Lux AHL and 3 uM of Rhl AHL. On the right, the system is locked into the red state after a pulse of 4 uM of Lux AHL and 4 uM of Rhl AHL. The model nicely shows the dominance of either TetR or LacI for the green, respectively red state.
