IGEM:Cambridge/2008/Notebook/Voltage/Output

From 2008.igem.org

Revision as of 17:01, 29 October 2008 by Ellis (Talk | contribs)

Home   Voltage   Signalling   Bacillus   Modelling   Protocols   People  
Overview   Constructs   Mutants   Potassium Assay   Voltage Output   Future Work  

x

Setup

Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise.

  • Cells containing biobrick K090002 are grown overnight in NOK with 5%(by vol.) 2M KCl added - final 100mM K+.
  • Samples are then spun down in individual 1.5ml eppendorfs.

Experiment

  • Resuspend cells in 200μL clean NOK and place in sample wells
  • Lower probe electrode into cell solution and wait for stable reading - 1min.
  • Add sample solution

Apparatus setup

Results

Output at different GluR0 concentrations

x

Retrieved from "http://2008.igem.org/IGEM:Cambridge/2008/Notebook/Voltage/Output"