IGEM:Cambridge/2008/Notebook/Voltage/K+ Growth

From 2008.igem.org

Revision as of 17:49, 29 October 2008 by Ellis (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Home Voltage Signalling Bacillus Modelling Protocols People

Overview Constructs Mutants Potassium Assay Voltage Output Future Work

x

Method

For each of the 9 mutants, 3 [K+] of growth medium were examined: 1mM, 50mM & 200mM

Composition of Each Cuvette:

                     * 100ul cells
                     * 100ul KCl (10x stocks)
                     * 800ul LB 
                       ----------------------
                         1ml Final Volume

The spectrophotometer was zeroed using LB.
OD readings were taken every 30 minutes for 3 hours.
In between readings, cultures were stored at room temperature & covered with parafilm.

Results

Link to raw OD600 Data in Excel

x