Setup

• Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise.
• Oscilloscope is set to 0.2V/div and 0.2 sec/div
• Cells containing biobrick K090002 are grown overnight in NOK with 5%(by vol.) 2M KCl added - final 100mM K+.
• Samples are then spun down in individual 1.5ml eppendorfs.

Experiment

• Resuspend cells in 200μL clean NOK and place in sample wells
• Lower probe electrode into cell solution and wait for stable reading - 1min.
• Add sample solution

Results

• With the setup above a voltage change could be observed on the oscilloscope.
• The following results were recorded by adding different amounts of 10uL glutamate to the samples.

 *10uL Aspartate produced the bottom curve, showing very little recover
 *5uL Glutamate gave the next curve
 *10uL Glutamate gave the third curve
 *20uL Glutamate gave the best recovery, reaching the highest level