Alberta NINT/23 June 2008

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lab work (SD): 

            Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008).

lab work (JD): 

             Quantitated K102005/B+N              
             Ligated K102005/B+N with the newly annealed TA0/B+N=K102007
             Transformed XL1-B cells with K102007
             Plated cells on LB+Amp50
             From sequencing K102003.4 becomes official K102003.

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