User:UW iGEM Team/25 June 2008

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Yeast Shuttle Plasmid

-Obtained plate of yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0)

-Incubated yeast at 30 degrees Celsius overnight