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Team:NYMU-Taipei/Project/Phosphate/PRIMER of pstSCAB-phoU PST operon
From 2008.igem.org
Revision as of 20:03, 29 October 2008 by Blackrabbit (Talk | contribs)
Contents |
pst Operon
operon structure
biobrick cutting sites scanning report
- by serial cloner 1.3
>EG10734 pstS (complement(3909548..3904876) E. coli atgAAAGTTA TGCGTACCAC CGTCGCAACT GTTGTCGCCG CGACCTTATC GATGAGTGCT TTCTCTGTGT TTGCAGAAGC AAGCCTGACA GGTGCAGGTG CAACCTTCCC TGCGCCGGTG TATGCCAAAT GGGCTGACAC TTACCAGAAA GAAACCGGTA ATAAAGTTAA CTACCAGGGT ATCGGTTCTT CCGGTGGCGT AAAACAGATT ATCGCTAATA CCGTTGATTT TGGTGCCTCT GACGCGCCGC TGTCTGACGA AAAACTGGCT CAGGAAGGTC TGTTCCAGTT CCCGACCGTG ATTGGCGGCG TGGTGCTGGC GGTTAACATT CCAGGGCTGA AGTCTGGCGA ACTGGTGCTG GATGGTAAAA CCCTCGGCGA CATCTACCTG GGCAAAATCA AGAAGTGGGA TGATGAAGCC ATCGCCAAAC TGAATCCGGG TCTGAAACTG CCTTCACAAA ACATTGCTGT AGTACGCCGC GCAGATGGCT CCGGGACTTC CTTCGTCTTC ACCAGCTACC TGGCGAAAGT GAACGAAGAG TGGAAAAACA ACGTTGGTAC TGGCTCTACC GTAAAATGGC CGATCGGTCT GGGCGGTAAA GGTAACGACG GTATCGCCGC GTTCGTTCAG CGTCTGCCGG GTGCAATTGG TTATGTTGAA TATGCTTACG CGAAGCAGAA CAACCTGGCG TACACCAAAC TGATCTCCGC TGATGGTAAA CCGGTTAGTC CGACCGAAGA AAACTTCGCT AATGCAGCAA AAGGTGCAGA CTGGAGCAAA ACCTTCGCTC AGGATCTGAC CAACCAGAAA GGCGAAGATG CATGGCCTAT TACCTCTACC ACGTTCATTC TGATCCACAA AGATCAGAAG AAACCAGAAC AAGGCACAGA AGTGCTGAAA TTCTTCGACT GGGCGTACAA AACCGGGGCT AAACAGGCGA ACGACCTGGA TTACGCCAGC CTGCCGGATA GTGTAGTTGA ACAGGTTCGC GCTGCGTGGA AGACCAATAT TAAAGACAGT AGCGGTAAGC CGCTGTACTA ATAAAACTCC AGGCCGGGTA CGGTGTTTTA CGCCGCATCC GGCATTACAA AATGACTTTG TAAACGCGTT TAACTGAAGA GTAACTTATG GCTGCAACCA AGCCTGCTTT TAACCCACCG GGTAAAAAGG GCGACATAAT TTTCAGCGTG CTGGTAAAAC TGGCGGCGCT GATTGTGCTA TTGATGTTGG GTGGCATTAT TGTCTCTCTG ATCATCTCCT CCTGGCCGAG CATTCAGAAA TTTGGTCTGG CTTTCCTATG GACCAAAGAG TGGGATGCAC CGAACGATAT CTACGGGGCG CTGGTGCCGA TCTACGGTAC GTTGGTGACT TCGTTTATCG CGCTGCTGAT CGCCGTCCCG GTGAGTTTCG GTATCGCCCT GTTCCTGACT GAGCTTGCGC CTGGCTGGCT GAAACGCCCG CTGGGTATCG CCATTGAGCT GCTGGCAGCC ATTCCAAGTA TCGTTTACGG CATGTGGGGC CTGTTTATCT TTGCGCCGCT GTTCGCCGTT TACTTTCAGG AGCCGGTCGG CAATATCATG TCGAATATCC CGATTGTTGG CGCGCTGTTC TCTGGCCCCG CATTTGGTAT CGGTATCCTC GCGGCAGGCG TGATCCTCGC CATCATGATT ATTCCGTACA TTGCGGCGGT AATGCGTGAT GTGTTCGAAC AAACCCCGGT GATGATGAAA GAGTCGGCCT ACGGTATTGG CTGCACCACC TGGGAAGTTA TCTGGCGTAT CGTTCTTCCG TTCACCAAAA ATGGTGTTAT CGGCGGCATC ATGCTGGGGC TGGGCCGCGC GCTCGGTGAA ACCATGGCGG TGACCTTTAT CATCGGTAAC ACCTACCAGC TCGACAGCGC CTCGCTGTAT ATGCCGGGCA ACAGTATCAC CTCTGCGCTG GCGAACGAAT TTGCGGAAGC GGAATCCGGT CTGCACGTTG CCGCACTGAT GGAACTGGGC CTGATCCTGT TTGTGATTAC CTTCATCGTC CTCGCCGCAT CGAAGTTTAT GATTATGCGC CTGGCTAAGA ATGAGGGGGC ACGCTAATGG CTATGGTTGA AATGCAAACC ACTGCGGCGC TGGCTGAATC TCGCCGCAAA ATGCAGGCGC GTCGCCGCCT CAAAAACCGT ATTGCGCTGA CGCTCTCGAT GGCGACGATG GCCTTCGGCC TGTTCTGGCT GATCTGGATT TTAATGTCCA CCATCACTCG CGGTATCGAC GGTATGTCGC TGGCGCTGTT CACTGAAATG ACGCCGCCGC CCAATACGGA AGGTGGTGGT CTGGCGAACG CTCTGGCGGG TAGCGGGCTG TTAATTTTGT GGGCCACGGT ATTCGGTACG CCGCTGGGCA TTATGGCGGG GATTTATCTG GCGGAATATG GTCGTAAATC CTGGCTGGCA GAAGTGATTC GCTTCATTAA CGACATTCTG CTCTCTGCGC CGTCGATTGT GGTTGGTCTG TTTGTTTACA CCATTGTGGT GGCGCAGATG GAGCACTTCT CCGGCTGGGC GGGCGTGATT GCCCTGGCGT TGTTGCAGGT GCCGATTGTT ATCCGCACCA CCGAGAACAT GCTGAAACTG GTGCCGTACA GCCTGCGTGA AGCGGCTTAT GCGCTGGGTA CACCGAAGTG GAAGATGATC TCTGCGATTA CGCTGAAAGC GTCGGTGTCC GGGATTATGA CCGGTATCCT GCTGGCGATT GCCCGTATTG CTGGTGAAAC CGCGCCGCTG CTGTTTACCG CGCTCTCCAA CCAGTTCTGG AGCACGGACA TGATGCAGCC GATCGCCAAC CTGCCGGTGA CGATCTTTAA GTTTGCGATG AGCCCGTTTG CGGAATGGCA GCAATTGGCC TGGGCCGGGG TATTGATCAT TACCCTGTGC GTACTGCTGC TGAACATTCT GGCGCGCGTT GTTTTTGCGA AGAATAAACA CGGTTGATAT TGCTGACACG GTTTTCCCCT CACCCTAACC CTCTCCCCAG AGGGGCGAGG GGACCGACCG AGCGCCTTTT TGACTCTGTA CACGGTTAAC ACTTTGCCGG ATGCGGCGTG AACGCCTGAT CCGGCCTACG GTAAGCCTGA TTAGCGAAGT GCATCAGGCA CGATGAGGAA AAGATTGCAA TGAGTATGGT TGAAACTGCC CCGAGTAAAA TTCAGGTTCG TAATTTGAAC TTCTACTACG GCAAATTCCA TGCCCTGAAA AACATCAACC TGGATATCGC TAAAAACCAG GTAACGGCGT TTATCGGGCC GTCCGGCTGC GGTAAATCGA CGCTGCTGCG TACCTTCAAC AAAATGTTTG AACTGTACCC GGAGCAGCGT GCGGAAGGTG AAATTCTGCT TGATGGCGAC AACATCCTGA CCAACTCTCA GGATATCGCA CTGCTGCGTG CGAAAGTGGG CATGGTGTTC CAGAAACCGA CGCCGTTTCC GATGTCCATC TACGACAACA TCGCTTTTGG CGTTCGTCTG TTTGAGAAGC TCTCCCGTGC CGACATGGAC GAGCGCGTGC AGTGGGCATT GACCAAAGCC GCATTGTGGA ACGAAACCAA AGATAAATTG CACCAGAGCG GTTACTCTCT CTCTGGTGGT CAGCAACAGC GTCTGTGTAT TGCGCGTGGT ATCGCCATTC GCCCGGAAGT GCTGCTGCTC GACGAACCGT GTTCGGCGCT CGACCCTATC TCTACCGGGC GTATTGAAGA GCTGATCACC GAACTGAAGC AGGATTACAC CGTGGTGATC GTCACCCACA ACATGCAGCA GGCTGCGCGT TGTTCCGACC ACACGGCGTT TATGTACCTG GGCGAATTGA TTGAGTTCAG CAACACGGAC GATCTGTTCA CCAAGCCAGC GAAGAAACAA ACAGAAGACT ACATCACCGG TCGTTACGGT TGATTCAGGA GTGCGTTATG GACAGTCTCA ATCTTAATAA ACATATTTCC GGCCAGTTCA ACGCCGAACT GGAAAGTATC CGCACGCAGG TGATGACCAT GGGCGGCATG GTGGAGCAGC AGCTTTCTGA TGCAATCACC GCGATGCATA ACCAGGACAG CGATCTGGCG AAGCGCGTCA TCGAAGGCGA CAAGAACGTC AACATGATGG AAGTGGCGAT CGATGAAGCC TGCGTGCGCA TTATCGCCAA ACGTCAGCCG ACGGCGAGCG ACCTGCGACT GGTTATGGTG ATCAGTAAAA CCATTGCCGA GCTGGAGCGT ATTGGCGACG TGGCGGACAA AATCTGCCGT ACTGCGCTGG AGAAATTCTC CCAGCAGCAT CAGCCGTTGC TGGTAAGTCT GGAGTCGCTG GGCCGTCATA CCATCCAGAT GCTGCACGAC GTGCTGGACG CGTTCGCGCG GATGGACATT GACGAAGCGG TACGTATTTA TCGTGAAGAT AAAAAAGTCG ATCAGGAATA CGAAGGTATT GTTCGTCAAC TGATGACCTA CATGATGGAA GATTCGCGTA CCATTCCGAG CGTACTTACT GCGCTGTTCT GCGCGCGTTC TATCGAACGT ATTGGCGACC GCTGCCAGAA TATTTGTGAG TTTATCTTCT ACTACGTGAA GGGGCAGGAT TTCCGTCACG TCGGTGGCGA TGAGCTGGAT AAACTGCTGG CGGGGAAAGA TAGCGACAAA TAA
- A overlap between pstA and pstC
pstS - pst Operon Forward Primer
>EG10734 pstS (complement(3909548..3908508)) E. coli
atgAAAGTTA TGCGTACCAC CGTCGCAACT GTTGTCGCCG CGACCTTATC GATGAGTGCT
TTCTCTGTGT TTGCAGAAGC AAGCCTGACA GGTGCAGGTG CAACCTTCCC TGCGCCGGTG
TATGCCAAAT GGGCTGACAC TTACCAGAAA GAAACCGGTA ATAAAGTTAA CTACCAGGGT
ATCGGTTCTT CCGGTGGCGT AAAACAGATT ATCGCTAATA CCGTTGATTT TGGTGCCTCT
GACGCGCCGC TGTCTGACGA AAAACTGGCT CAGGAAGGTC TGTTCCAGTT CCCGACCGTG
ATTGGCGGCG TGGTGCTGGC GGTTAACATT CCAGGGCTGA AGTCTGGCGA ACTGGTGCTG
GATGGTAAAA CCCTCGGCGA CATCTACCTG GGCAAAATCA AGAAGTGGGA TGATGAAGCC
ATCGCCAAAC TGAATCCGGG TCTGAAACTG CCTTCACAAA ACATTGCTGT AGTACGCCGC
GCAGATGGCT CCGGGACTTC CTTCGTCTTC ACCAGCTACC TGGCGAAAGT GAACGAAGAG
TGGAAAAACA ACGTTGGTAC TGGCTCTACC GTAAAATGGC CGATCGGTCT GGGCGGTAAA
GGTAACGACG GTATCGCCGC GTTCGTTCAG CGTCTGCCGG GTGCAATTGG TTATGTTGAA
TATGCTTACG CGAAGCAGAA CAACCTGGCG TACACCAAAC TGATCTCCGC TGATGGTAAA
CCGGTTAGTC CGACCGAAGA AAACTTCGCT AATGCAGCAA AAGGTGCAGA CTGGAGCAAA
ACCTTCGCTC AGGATCTGAC CAACCAGAAA GGCGAAGATG CATGGCCTAT TACCTCTACC
ACGTTCATTC TGATCCACAA AGATCAGAAG AAACCAGAAC AAGGCACAGA AGTGCTGAAA
TTCTTCGACT GGGCGTACAA AACCGGGGCT AAACAGGCGA ACGACCTGGA TTACGCCAGC
CTGCCGGATA GTGTAGTTGA ACAGGTTCGC GCTGCGTGGA AGACCAATAT TAAAGACAGT
AGCGGTAAGC CGCTGTACTA A
- Annealing - len:21, Tm:54, GC%:43
- GC GAATTCGCGGCCGCTTCTAG atgAAAGTTA TGCGTACCAC C
- Total length - 43 (2 + 20 + 21)
phoU - pst Operon Reverse Primer
>EG10735 phoU (complement(3905601..3904876)) E. coli
atgGACAGTC TCAATCTTAA TAAACATATT TCCGGCCAGT TCAACGCCGA ACTGGAAAGT
ATCCGCACGC AGGTGATGAC CATGGGCGGC ATGGTGGAGC AGCAGCTTTC TGATGCAATC
ACCGCGATGC ATAACCAGGA CAGCGATCTG GCGAAGCGCG TCATCGAAGG CGACAAGAAC
GTCAACATGA TGGAAGTGGC GATCGATGAA GCCTGCGTGC GCATTATCGC CAAACGTCAG
CCGACGGCGA GCGACCTGCG ACTGGTTATG GTGATCAGTA AAACCATTGC CGAGCTGGAG
CGTATTGGCG ACGTGGCGGA CAAAATCTGC CGTACTGCGC TGGAGAAATT CTCCCAGCAG
CATCAGCCGT TGCTGGTAAG TCTGGAGTCG CTGGGCCGTC ATACCATCCA GATGCTGCAC
GACGTGCTGG ACGCGTTCGC GCGGATGGAC ATTGACGAAG CGGTACGTAT TTATCGTGAA
GATAAAAAAG TCGATCAGGA ATACGAAGGT ATTGTTCGTC AACTGATGAC CTACATGATG
GAAGATTCGC GTACCATTCC GAGCGTACTT ACTGCGCTGT TCTGCGCGCG TTCTATCGAA
CGTATTGGCG ACCGCTGCCA GAATATTTGT GAGTTTATCT TCTACTACGT GAAGGGGCAG
GATTTCCGTC ACGTCGGTGG CGATGAGCTG GATAAACTGC TGGCGGGGAA AGATAGCGAC
AAATAA
- Annealing - len:22, Tm:54, GC%:41
- GC CTGCAGCGGCCGCTACTAGTA GGGGAA AGATAGCGAC AAATAA
- Total length - 44 (2 + 20 + 22)
