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User:University of Washington/25 June 2008
From 2008.igem.org
Yeast Shuttle Plasmid
- An agar plate containing yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) was obtained.
- The yeast was incubated overnight at 30 degrees Celsius.
LuxR under AraC and TetR
- Obtained BBa_R0040 (TetR), BBa_R0080 (AraC)
- transformed and plated with Amp
LuxR under pLac
- Obtained parts R0010, I0462, and J04430.
- R0010 and I0462 were transformed into MG strains, and plated on Amp.
- J04430 was transformed into DH strain, and plated on Amp.
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