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IGEM:Cambridge/2008/Notebook/Voltage/Future Work
From 2008.igem.org
Contents |
Future Work
Better K+ uptake
If a stronger K+ uptake could be made then in pricipal cells could be grown in the same medium as testing was done
- Up regulate KDP constitutively
- Find K+ tolerant strains
- Check maximum trans-membrane potential
Move to Bacillus
Need to make GluR0 Bacillus compatible
- Codon bias already favourable for Bacillus
- Need to put different promoter and RBS
- Integrate into genome?
Voltage Gated Potassium Channel
To get self propogating signal we need to find and construct a voltage gated potassium channel.
- Relatively rare in Prokaryotes
- Some available although not well characterised, such as Kch
- Overexpress and see if it improves output.
