Edinburgh/24 July 2008

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Edinburgh iGEM 2008

June
M	T	W	T	F	S	S
						[http://2008.igem.org/wiki/index.php?title=Edinburgh/1_June_2008&action=edit 1]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_June_2008&action=edit 2]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/3_June_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/4_June_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/5_June_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/6_June_2008&action=edit 6]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/7_June_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/9_June_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/10_June_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/11_June_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/12_June_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/13_June_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/14_June_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/16_June_2008&action=edit 16]	[http://2008.igem.org/Edinburgh/17_June_2008 17]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/18_June_2008&action=edit 18]	[http://2008.igem.org/Edinburgh/19_June_2008 19]	[http://2008.igem.org/Edinburgh/20_June_2008 20]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/21_June_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_June_2008&action=edit 23]	[http://2008.igem.org/Edinburgh/24_June_2008 24]	[http://2008.igem.org/Edinburgh/25_June_2008 25]	[http://2008.igem.org/Edinburgh/26_June_2008 26]	[http://2008.igem.org/Edinburgh/27_June_2008 27]	[http://2008.igem.org/Edinburgh/28_June_2008 28]	[http://2008.igem.org/Edinburgh/29_June_2008 29]
[http://2008.igem.org/Edinburgh/30_June_2008 30]

July
M	T	W	T	F	S	S
	[http://2008.igem.org/Edinburgh/1_July_2008 1]	[http://2008.igem.org/Edinburgh/2_July_2008 2]	[http://2008.igem.org/Edinburgh/3_July_2008 3]	[http://2008.igem.org/Edinburgh/4_July_2008 4]	[http://2008.igem.org/Edinburgh/5_July_2008 5]	[http://2008.igem.org/Edinburgh/6_July_2008 6]
[http://2008.igem.org/Edinburgh/7_July_2008 7]	[http://2008.igem.org/Edinburgh/8_July_2008 8]	[http://2008.igem.org/Edinburgh/9_July_2008 9]	[http://2008.igem.org/Edinburgh/10_July_2008 10]	[http://2008.igem.org/Edinburgh/11_July_2008 11]	[http://2008.igem.org/Edinburgh/12_July_2008 12]	[http://2008.igem.org/Edinburgh/13_July_2008 13]
[http://2008.igem.org/Edinburgh/14_July_2008 14]	[http://2008.igem.org/Edinburgh/15_July_2008 15]	[http://2008.igem.org/Edinburgh/16_July_2008 16]	[http://2008.igem.org/Edinburgh/17_July_2008 17]	[http://2008.igem.org/Edinburgh/18_July_2008 18]	[http://2008.igem.org/Edinburgh/19_July_2008 19]	[http://2008.igem.org/Edinburgh/20_July_2008 20]
[http://2008.igem.org/Edinburgh/21_July_2008 21]	[http://2008.igem.org/Edinburgh/22_July_2008 22]	[http://2008.igem.org/Edinburgh/23_July_2008 23]	[http://2008.igem.org/Edinburgh/24_July_2008 24]	[http://2008.igem.org/Edinburgh/25_July_2008 25]	[http://2008.igem.org/Edinburgh/26_July_2008 26]	[http://2008.igem.org/Edinburgh/27_July_2008 27]
[http://2008.igem.org/Edinburgh/28_July_2008 28]	[http://2008.igem.org/Edinburgh/29_July_2008 29]	[http://2008.igem.org/Edinburgh/30_July_2008 30]	[http://2008.igem.org/Edinburgh/31_July_2008 31]

August
M	T	W	T	F	S	S
				[http://2008.igem.org/Edinburgh/1_August_2008 1]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_August_2008&action=edit 2]	[http://2008.igem.org/Edinburgh/3_August_2008 3]
[http://2008.igem.org/Edinburgh/4_August_2008 4]	[http://2008.igem.org/Edinburgh/5_August_2008 5]	[http://2008.igem.org/Edinburgh/6_August_2008 6]	[http://2008.igem.org/Edinburgh/7_August_2008 7]	[http://2008.igem.org/Edinburgh/8_August_2008 8]	[http://2008.igem.org/Edinburgh/9_August_2008 9]	[http://2008.igem.org/Edinburgh/10_August_2008 10]
[http://2008.igem.org/Edinburgh/11_August_2008 11]	[http://2008.igem.org/Edinburgh/12_August_2008 12]	[http://2008.igem.org/Edinburgh/13_August_2008 13]	[http://2008.igem.org/Edinburgh/14_August_2008 14]	[http://2008.igem.org/Edinburgh/15_August_2008 15]	[http://2008.igem.org/Edinburgh/16_August_2008 16]	[http://2008.igem.org/Edinburgh/17_August_2008 17]
[http://2008.igem.org/Edinburgh/18_August_2008 18]	[http://2008.igem.org/Edinburgh/19_August_2008 19]	[http://2008.igem.org/Edinburgh/20_August_2008 20]	[http://2008.igem.org/Edinburgh/21_August_2008 21]	[http://2008.igem.org/Edinburgh/22_August_2008 22]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_August_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/24_August_2008&action=edit 24]
[http://2008.igem.org/Edinburgh/25_August_2008 25]	[http://2008.igem.org/Edinburgh/26_August_2008 26]	[http://2008.igem.org/Edinburgh/27_August_2008 27]	[http://2008.igem.org/Edinburgh/28_August_2008 28]	[http://2008.igem.org/Edinburgh/29_August_2008 29]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/30_August_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=Edinburgh/31_August_2008&action=edit 31]

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Week 6

Thursday 24 July 08

Purified PCR products P28 (rbs+crtB) and P29 (rbs+crtI). (OG)
Double digestions using XbaI/PstI (buffer H) to add P12 (rbs+dxs), P15 (rbs+appY), P28 (rbs+crtB) and P29 (rbs+crtI) to Edinbrick1. (Yan, OG)
Double digestion using SacI/SpeI (BufferE) to add P31 (P_zntA) to Edinbrick1. (Yan, OG)
Purified genomic DNA from C. fimi culture (made from plate 37, incubated in 25ml bottle at 30C for 48 hours with shaking). Used Promega Wizard Genomic DNA Purification Kit, lysozyme and RNAse+EDTA (as replacement for proprietary RNAse Solution, of which none was left). Final solution left overnight in refrigerator at 4°C. (AM)
Plated C. fimi culture from plate 37...
- Incubated in 25ml bottle for 48 hours at 30°C with shaking ... to Plate 48.
- Incubated in 50ml bottle for 24 hours at 30°C with shaking ... to Plate 49.
- Incubated in 250ml flask for 24 hours at 30°C with shaking ... to Plate 50. (AM)
PCR of cenA (P32), cenB (P33), cenC (P34), cex (P35), annealing 1 min 55°C, extension 90 sec 70°C. Run on Gel 24 - unsuccessful. (AM)

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