Team:Imperial College/CAT

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Chloramphenicol Acetyltransferase Characterisation

Characterisation of the chloramphenicol concentrations at which chlorampehnicol acetyltransferase ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J31005 Part: BBa_J31005]) (CAT) will protect cells, allowing them to grow and thrive is essentially to allow the correct selection when using the CAT gene.

If a culture of cells becomes contaminated with another chloarmphenicol resistant bacterium and we lack an extra resistance marker, the upper limit at which our cells can survive in chloramphenicol may allows the removal of the contaminating strain or species.

To this end, characterisation of chloramphenicol acetyl transferase was carried out according to this protocol

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