This year Imperial College has added 45 new B.subtilis specific parts to the registry. In addition we sort to characterise some of these parts, including the constitutive promoter and ribosome binding site pveg-spoVG. The testing constructs we developed for this are shown below:
Summary of Data
The promoter and RBS combinations were characterised by measuring the steadty-state levels expression of fluorescent proteins in B.subtilis. Cultures of B.subtilis transformed with the test constructs and non-transformed B.subtilis (control) were grown to the mid-log phase. Fluorescence and O.D.600 were both measured using a plate reader to generate fluorescence levels of the various samples. This fluorescence data was normalised based on cell density by using the O.D.600 and a calibration curve of O.D.600 vs colony forming units
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