"
Chemotaxis Modeling
From 2008.igem.org
| HOME | Team | Project 1 | Project 2 | Parts | Modelling | Notebook | Doodle Board |
|---|
Equations and Parameters
Tables and Charts
Figure. Simulation result of Bacteria movement of CdLocalizer Project. Horizontal axis is x coordinate of bacteria while vertical axis is time. The change in color illustrates the density of bacteria.Deep Red is the most while blue is none.In this chart the bacteria moves toward a certain concentration of designated chemical.
Figure. Simulation result of Bacteria movement of DualReceptor Project. Horizontal axis is x coordinate of bacteria while vertical axis is time. The change in color illustrates the density of bacteria.Deep Red is the most while blue is none.In this chart the bacteria moves toward a certain concentration of designated chemical.
These figures show the x coordinate of bacteria in a time sequence from 0s to 10000s. Bacterial chemotaxis behaviors are simulated in a mountain-shaped concentration field. The peak value of the concentration field is 20 and size constant of it is 20 mm. The chemotaxis effects of theophylline chemotaxis design and pseudochemotaxis are compared here. Pseudochemotaxis model derives from theophylline model via setting copy number of LacI plasmid at 0. Bacterium motilities of the two models are regulated to the same level to make them comparable. Top two figures are box plots which show the distribution of x coordinate. The box has lines at the lower quartile, median, and upper quartile x values of bacterium population. The whiskers extending from each end of the box show the extent of the rest of x coordinate value. Outliers marked by red plus are bacteria with x values beyond the ends of the whiskers. The contents of two figures at the bottom are similar to those above. They show the x-t curves of each bacterium.
These results illustrate that the chemotaxis effects is not improved significantly after adding a feed back loop to the pathway. Therefore, we should think about a question: what is the sufficient condition for a pathway to direct chemotaxis well. Some conditions learned from WT chemotaxis pathway are necessary, which means chemotaxis behavior will be harmed if they are not fulfilled.
Our following research focus on how to find a design principles of chemotaxis pathway. Actually, functions of chemotaxis pathway involve two aspects: signal transduction and signal conversion. It not only bring ligand concentration signal to molecule motor, but also calculates the signal in order to make motor work in right state. Cybernetics provides an idea that we should design the quantity relationship between input and output before considering pathway realization. We analyze dynamic properties of WT chemotaxis pathway and design linear controller according to that. After finding a good controller, we start to design novel chemotaxis pathway basing on it.
| HOME | Team | Project 1 | Project 2 | Parts | Modelling | Notebook | Doodle Board |
|---|
