Landing Pads: aid in the insertion of biological constructs onto the chromosome of E. coli

Landing Pad Goals:

• BioBrick compatibility
• Easy phenotypic screening
• Easy sequencing
• Limit noise

Polylinker Features:

• BioBrick compatible restriction sites - these allow for standard BioBrick construction in forward and reverse directions
• Additional building restriction sites - these are for building the landing pad, as well as changing the homologous regions and drug resistance gene (can also be used for building if needed)
• Bar code sequence - a 20 bp segment that allows for easy sequencing
• Drug resistance gene - this allows for easy phenotypic screening
• Homologous regions - these specify where on the chromosome you wish to put your constructs.

stuff about landing pad plasmids