Team:Chiba/Calendar-Home/14 September 2008

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13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep


Transformation
Competent cells : XL10Gold
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
--->(15/9)


Team:Output

Time Responce Liquid

Digestion test

  • [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
  • [http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
  • [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
  • [http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
  • [http://partsregistry.org/Part:BBa_I716452 BBa_I716452]
  • [http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
  • [http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
  • [http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
  • [http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](×2)
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
Sample No. each
DNA(digested sample)(μL) 3
Buffer2(x10)(μL) 1
dH2O(μL) 6
EcoRI(μL) 0.075
TOTAL(μL) 10

--> left at 37°C for 1 hour

Gel check

Sample No. each
DNA(digestion sample)(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12
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