Future Work

Better K+ uptake

If a stronger K+ uptake could be made then in principal cells could be grown in the same medium as testing was done. This would potentially allow cells to be grown as a surface over an agar plate and still act to produce action potentials, thereby acting as neurones.

• Up regulate KDP constitutively
• Find K+ tolerant strains
• Check maximum trans-membrane potential

Move to Bacillus

Need to make GluR0 Bacillus compatible

• Codon bias already favourable for Bacillus
• Need to put different promoter and RBS
• Integrate into genome?

Voltage Gated Potassium Channel

To get self propagating signal we need to find and construct a voltage gated potassium channel.

• Relatively rare in Prokaryotes
• Some available although not well characterised, such as Kch
• Overexpress and see if it improves output.