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Minnesota/24 June 2008
From 2008.igem.org
Revision as of 15:40, 27 June 2008 by Emartin9808 (Talk | contribs)
| 1. Gel Electrophoresis: Used this technique to show plasmid DNA sequence. Materials: |
| a. 50 uL of 1% agarose gel |
| b. Buffer TAE |
| c. One gram of 1% agarose per 100 uL of TAE |
| d. Ethidium bromide (intercalating agent) |
| Problem encountered: electrophoretic gels with 1% agarose had deficient wells |
| Solution: add 0.5 grams more of agarose to the 100uL of TAE buffer |
