Alberta NINT/27 June 2008
From 2008.igem.org
lab work (SD):
Digested K102002 and K102008.1 with EcoRI + PstI (again) Incubated at 37 C for 1 hr Ran on 2% agarose gel at 110V for 50 min Excised small fragments from the gel and put them at -20 C for the long weekend