All the parts submitted by last year's team can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=iGEM2007_Alberta here].
The summer began with working on the butanol project as a training exercise to get the team familiarized with lab procedures. The team worked on ligating individual butanol inserts into the J61003 and I0500 vectors. Furthermore, protein expressions were to be done on these plasmids once the ligations were successfully completed. The ligations were a success by the beginning of July.
The team proceeded to show protein expression of the individual butanol inserts. The Western blots were successfully completed by the end of July.
Finally, with the eagerness of the team to complete last year's project, the completion of the full butanol operon was adopted as a side project. Last year's intended operon (from insert 21 to insert 25) was successfully assembled in PSB1A3 by mid-October!
Last year's intended operon included the butanol inserts:
RBS + B-hydroxy butyryl CoA dehydrogenase
RBS + Enoyl-CoA hydratase
RBS + Butyryl CoA dehydrogenase
RBS + Butyraldehyde dehydrogenase
RBS + Butanol dehydrogenase
The full optimal butanol operon requires three addition inserts:
to overexpress butanol production. It is expected that ETFA and ETFB are required along with the five original butanol inserts to produce butanol. Thiolase will help overexpress the production of butanol.
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