Team:Davidson-Missouri Western/Experience Gained on New Registry Parts

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Cell-to-cell signaling by XOR gate constructs - The ability of two different XOR gate constructs to send a signal via the Lux system was measured by coplating bacterial XOR clones with bacteria containing a Lux receiver construct.

Positive control using a Lux sender and a Lux receiver (K09100) Green fluorescence indicates LuxI expression in the Lux sender and receipt of the intercellular signal by the receiver.

9100 Sender.JPG


XOR gate construct [http://partsregistry.org/wiki/index.php?title=Part:BBa_K091173 K091173Q1] coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.

9100 K82.JPG


XOR gate construct [http://partsregistry.org/wiki/index.php?title=Part:BBa_K091182 K091182Q1] coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.

9100 K73.JPG


[http://partsregistry.org/Part:BBa_K091112:Experience Improved pLac promoter called pLacIQ1]

Trial1.png


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