Team:Paris/constructions
From 2008.igem.org
Model constructions: from the modelling to the characterization
Our project BacterioClock is based on an oscillating FIFO synchronised at the population level. To obtain and have preliminary results of this system we divided it in three "little" modules as the modelling team exposed them previously.
- The First one aims to see the FIFO which constitute our core system.
A simple manner to see it, is to implement one class II promotor followed by a reporter gene like GFP in a bacteria strain that is able to produce the flagella. By this way, we are able to see in single cell the order of the activation of pFliL then pFlgA then pFlhB and then the inactivation in the same order as a FIFO.