Team:MIT/Notebook

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Procedural Daily Notebook

Please see our daily notebook for a detailed daily summary of our daily meetings, brainstorming, discussions, and progress.

[http://openwetware.org/wiki/MIT_iGEM_T4_and_Quick_Ligation DNA Ligations]
[http://openwetware.org/wiki/MIT_iGEM_Top10_ChemComp_Ecoli_transformation_protocol#Regular_Transformation_protocol_for_Top10_cells Top10 chem competent cell transformation]
[http://web.mit.edu/biopolymers/www/DNA.html DNA sequencing]
[http://openwetware.org/wiki/Endy_pcr PCR]
[http://openwetware.org/wiki/MIT_iGEM_Agarose_Gels Running DNA Gels]
[http://openwetware.org/wiki/MIT_iGEM_Restriction_Digests Restriction Digests]
[http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks Preparing Antibiotic Stocks]
Protein Purification

[http://www.biology.utah.edu/jorgensen/wayned/ape/ DNA Plasmid Editor]
[http://slam.bs.jhmi.edu/gd/ Peptide Sequence Manipulations]
Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
PMID 9920267
A synthetic peptide adhesion epitope as a novel antimicrobial agent.
PMID 9062560
Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
PMID 12788739
Determination of the domain of the Lactobacillus delbrueckii subsp. bulgaricus cell surface proteinase PrtB involved in attachment to the cell wall after heterologous expression of the prtB gene in Lactococcus lactis. (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
PMID 11772607
Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.

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