"
Template:Team:UC Berkeley/Notebook/CDB construction
From 2008.igem.org
Revision as of 21:39, 27 June 2008 by ChrisBrown (Talk | contribs)
Contents |
PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------------------------------- ca8373 Forward Biobricking of mRFP CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag ca8374 Reverse Biobricking of mRFP CGttaGGATCCttactcccccaagaaaatcc
pBca1256-K112400
Biobricking of promoter for grpE protein PCR grpE-F and grpE-R on MG1655 (129 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112400 ---------------------------- grpE-F Forward Biobricking cgataGAATTCatgAGATCTccgaggtccttgttgcgaag grpE-R Reverse Biobricking cgtagGGATCCcgcattcatctcgctaacttc
pBca1256-K112401
Biobricking of promoter for recA protein PCR recA-F and recA-R on MG1655 (317 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112401 ---------------------------- recA-F Forward Biobricking cgataGAATTCatgAGATCTagagaagcctgtcggcaccg recA-R Reverse Biobricking cgtagGGATCCcccagtgctgccgccaacgc
pBca1256-K112402
Biobricking of promoter for fabA protein PCR fabA-F and fabA-R on MG1655 (285 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112402 ---------------------------- fabA-F Forward Biobricking cgataGAATTCatgAGATCTggccattacgttggctgaac fabA-R Reverse Biobricking cgtagGGATCCgaagttaccacccgtttcgg
