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Protocols PCR
From 2008.igem.org
37ul H2O
5ul 10X PCR Buffer
1ul dNTP (10mM)
0.5ul Polymerase
0.5ul of each Primer (100uM)
1ul Template
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50ul
ThermoCycle Program
98*C.......30 sec
_____________
98*C.......10 sec
65*C.......30 sec
72*C.......(20 sec/kb {Min 20sec})
_____________
72*C.......5 min
10*C........Forever
Alternate ThermoCycle Program #1
90*C.......30 sec
_____________
92*C.......10 sec
62*C.......30 sec
72*C.......(20 sec/kb {Min 20sec})
_____________
72*C.......5 min
10*C........Forever
Alternate ThermoCycle Program #2
90*C.......30 sec
_____________
92*C.......10 sec
62*C.......60 sec
72*C.......(20 sec/kb {Min 20sec})
_____________
72*C.......5 min
10*C........Forever
