User:University of Washington/4 August 2008

From 2008.igem.org

LuxR from pLac

-R0010 and E0240 ligation was transformed into DH5a strain.

-Original R0010 and E0240 ligation (from two? weeks ago) that had an inconclusive sequencing was sent in for sequencing again.

-Part I0462 (receieved from igem hq) was sent in for sequencing.

MG1655Z1

- MG1655Z1 from section 1 and 5 were miniprepped. Glycerol stock were made for both.

LuxR from AraC and TetR

- Ran gel for P1010 (AC x 3, Kan x 3) to combine the DNA into one tube.

- Restriction digest the P1010 only one on pSB1AC3, and the PCR Elowitz's promoter D29 with XbaI and PstI.

  • mixed two reaction
dH2O(ul)NEB3(ul)BSA(ul)DNA(ul)XbaI(ul)PstI(ul)
P1010 (AC)32.550.51011
D2932.550.51011
  • incubated 37 degree Celcius about 1 hour
  • 20 mins in 80 degree Celcius water bath
  • stored in the fridge



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