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Melbourne/5 August 2008
From 2008.igem.org
Done today:
- Red light retest.
4 transformations:
- Cph8 + pPL-PCB
- Cph8
- PCB
- -ve control
Protocol:
- 2μL DNA
- RU1012
- 30 min ice
- 1 min 42°C
- 1 hour at 37°C + LB
Each transformation plated onto 2 plates, one of which is wrapped in foil. Placed in 37°C room at genetics which light on
Plates: Amp Kan Chlor Arabinose (2mM) IPTG (1mM) X-gal
