1. Plasmid Prep 2mL Ligated Product cultures from 07-14-2008. Follow the QIA prep and purify steps as seen in on 07-14-2008.
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2. Move Dual Promoter Plates from incubator to fridge. This will stop over growth and mutations before picking into 2mL cultures is performed later on.
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3. Transform paper DNA from iGEM notebook: (1) RFP, (2) TetR promoter, (3) Terminator, (4) MCherry. Follow iGEM handout for details on paper transformations into TOP10 competent cells.
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Example of Transformation using TetR Promoter Gene
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4. Sequencing Results: Sequencing results returned poor results. Redo/rework on sequences.
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