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Team:Caltech/Project/Vitamins/Parts
From 2008.igem.org
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Folate Production Parts
Basic Parts{{{{!}}}border="1" {{{!}}}+ Basic Parts (Extracted from L.lactis subspe. IL1403 genome) | Part Name | Registry # | Description | Cloned? | Sequence confirmed?
{{{!}}}- | Entire folate synthesis operon
{{{!}}} [http://partsregistry.org/Part:BBa_K137002 BBa_K137002]{{{!}}}{{{!}}} Includes folB+folKE+folP+ylgG+folC {{{!}}}{{{!}}} NO {{{!}}}{{{!}}} NO {{{!}}}- | folB
{{{!}}} [http://partsregistry.org/Part:BBa_K137009 BBa_K137009]{{{!}}}{{{!}}} dihydroneopterin aldolase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | folKE
{{{!}}} [http://partsregistry.org/Part:BBa_K137011 BBa_K137011]{{{!}}}{{{!}}} fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | folP
{{{!}}} [http://partsregistry.org/Part:BBa_K137012 BBa_K137012]{{{!}}}{{{!}}} Dihydropteroate synthase {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO {{{!}}}- | folC
{{{!}}} [http://partsregistry.org/Part:BBa_K137013 BBa_K137013]{{{!}}}{{{!}}} folate synthetase/polyglutamyl folate synthetase {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO {{{!}}}- | pabA
{{{!}}} [http://partsregistry.org/Part:BBa_K137005 BBa_K137005]{{{!}}}{{{!}}} para-aminobenzoate synthetase component II {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | pabB
{{{!}}} [http://partsregistry.org/Part:BBa_K137006 BBa_K137006]{{{!}}}{{{!}}} para-aminobenzoate synthetase component I {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- {{{!}}}} Construction Intermediates{{{{!}}} border="1" {{{!}}}+ Construction Intermediates: Adding b0034 (strong RBS) before each individual gene | Part Name | Registry # | Description | Cloned? | Sequence confirmed?
{{{!}}}- | folB + b0034
{{{!}}} [http://partsregistry.org/Part:BBa_S03957 BBa_S03957]{{{!}}}{{{!}}} RBS + dihydroneopterin aldolase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | folKE + b0034
{{{!}}} [http://partsregistry.org/Part:BBa_S03958 BBa_S03958]{{{!}}}{{{!}}} RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | folP + b0034
{{{!}}} [http://partsregistry.org/Part:BBa_S03959 BBa_S03959]{{{!}}}{{{!}}} RBS + Dihydropteroate synthase {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO {{{!}}}- | folC + b0034
{{{!}}} [http://partsregistry.org/Part:BBa_S03960 BBa_S03960]{{{!}}}{{{!}}} RBS + folate synthetase/polyglutamyl folate synthetase {{{!}}}{{{!}}} NO{{{!}}}{{{!}}} NO {{{!}}}- | pabA + b0034
{{{!}}} [http://partsregistry.org/Part:BBa_S03976 BBa_S03976]{{{!}}}{{{!}}} RBS + para-aminobenzoate synthetase component II {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | pabB + b0034
{{{!}}} [http://partsregistry.org/Part:BBa_S03977 BBa_S03977]{{{!}}}{{{!}}} RBS + para-aminobenzoate synthetase component I {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | b0034 + folB + b0034 + folKE
{{{!}}} [http://partsregistry.org/Part:BBa_S03961 BBa_S03961]{{{!}}}{{{!}}} Combining folB (with RBS) + folKE (with RBS) {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- {{{!}}}} {{{{!}}} border="1" {{{!}}}+ Construction Intermediates: Adding j23100 (constitutive promoter) to each gene (with RBS already) | Part Name | Registry # | Description | Cloned? | Sequence confirmed?
{{{!}}}- | folB + j23100
{{{!}}} [http://partsregistry.org/Part:BBa_S04032 BBa_S04032]{{{!}}}{{{!}}} Promoter(j23100) + RBS(b0034) + dihydroneopterin aldolase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | folKE + j23100
{{{!}}} [http://partsregistry.org/Part:BBa_S04033 BBa_S04033]{{{!}}}{{{!}}} Promoter + RBS + fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | pabA + j23100
{{{!}}} [http://partsregistry.org/Part:BBa_S04034 BBa_S04034]{{{!}}}{{{!}}} Promoter + RBS + para-aminobenzoate synthetase component II {{{!}}}{{{!}}} YES{{{!}}}{{{!}}} YES {{{!}}}- | folBKE + j23100
{{{!}}} [http://partsregistry.org/Part:BBa_S04035 BBa_S04035]{{{!}}}{{{!}}} Promoter + folB (with RBS) + folKE (with RBS) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}- | pabB + j23100
{{{!}}} [http://partsregistry.org/Part:BBa_S04039 BBa_S04039]{{{!}}}{{{!}}} Promoter + pabB {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}- | pabB + b0015
{{{!}}} [http://partsregistry.org/Part:BBa_S04038 BBa_S04038]{{{!}}}{{{!}}} pabB + b0015 terminator {{{!}}}{{{!}}} MAYBE {{{!}}}{{{!}}} WAITING {{{!}}}- {{{!}}}} Composite Parts{{{{!}}} border="1" {{{!}}}+ Composite Parts: Adding b0015 (double terminator) to complete constructs with promoter + RBS already | Part Name | Registry # | Description | Cloned? | Sequence confirmed?
{{{!}}}- | folB + b0015
{{{!}}} [http://partsregistry.org/Part:BBa_K137053 BBa_K137053]{{{!}}}{{{!}}} Promoter(j23100) + RBS(b0034) + folB (dihydroneopterin aldolase) + double terminator (b0015) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}- | folKE + b0015
{{{!}}} [http://partsregistry.org/Part:BBa_K137054 BBa_K137054]{{{!}}}{{{!}}} Promoter + RBS + folKE (fusion gene: GTP cyclohydrolase & 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase) + double terminator (b0015) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}- | pabA + b0015
{{{!}}} [http://partsregistry.org/Part:BBa_K137055 BBa_K137055]{{{!}}}{{{!}}} Promoter + RBS + pabA (para-aminobenzoate synthetase component II) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} YES {{{!}}}- | pabB + b0015
{{{!}}} [http://partsregistry.org/Part:BBa_K137109 BBa_K137109]{{{!}}}{{{!}}} Promoter + RBS + pabB (para-aminobenzoate synthetase component I) {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} NO {{{!}}}- | folBKE + b0015
{{{!}}} [http://partsregistry.org/Part:BBa_K137056 BBa_K137056]{{{!}}}{{{!}}} Promoter + RBS + folB + folKE + b0015 {{{!}}}{{{!}}} YES {{{!}}}{{{!}}} NO {{{!}}}- {{{!}}}}   |
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