Team:Chiba/jk/γ/week 1

From 2008.igem.org

>実験ログ
>出力班


Contents

WEEK 1

17,August 2008

PCR

  • [http://partsregistry.org/Part:BBa_E0430 BBa_E0430]①
  • [http://partsregistry.org/Part:BBa_E0420 BBa_E0420]②
  • [http://partsregistry.org/Part:BBa_J06702 BBa_J06702]③
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]④
  • BBa_I73003⑤
  • BBa_I73004⑥
  • BBa_I730026⑦
  • [http://partsregistry.org/Part:BBa_I763002 BBa_I763002]⑧
>
Sample No.
DNA tamplate 11111111
FW primer 55555555
VR primer 55555555
dNTPmix 1010101010101010
thermo pol buffer 1010101010101010
DNA pol(VENT) 11111111
dH2O 6868686868686868
TOTAL 100100100100100100100100


20,August 2008

Transformation

  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](Luciferase Renilla)-->colony
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019](Luciferase Fire fly)-->no colony
  • [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](LacZ)-->colony
  • [http://partsregistry.org/Part:BBa_E2030 BBa_E2030](Venus YFP)-->not so good
  • YFP(P-GEX)-->colony
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620]-->colony
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]-->colony


21,August 2008

Transformation

  • [http://partsregistry.org/Part:BBa_J32007 BBa_J32007](Lux CDABE)-->no colony
  • [http://partsregistry.org/Part:BBa_B0034 BBa_B0034](RBS)-->colony


22,August 2008

Mini prep

  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](Luciferase)
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]
  • [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](LacZ)
  • PUC19
  • PGEX VENUS YFP(GST fusion)


Transformation

  • PGEX VENUS YFP(GST fusion)


>>Next week


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