Team:Chiba/jk/γ/week 1
From 2008.igem.org
Contents |
WEEK 1
17,August 2008
- [http://partsregistry.org/Part:BBa_E0430 BBa_E0430]①
- [http://partsregistry.org/Part:BBa_E0420 BBa_E0420]②
- [http://partsregistry.org/Part:BBa_J06702 BBa_J06702]③
- [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]④
- BBa_I73003⑤
- BBa_I73004⑥
- BBa_I730026⑦
- [http://partsregistry.org/Part:BBa_I763002 BBa_I763002]⑧
Sample No. | ① | ② | ③ | ④ | ⑤ | ⑥ | ⑦ | ⑧ |
DNA tamplate | 1 | 1 | 1 | 1 | >1 | 1 | 1 | 1 |
FW primer | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
VR primer | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
dNTPmix | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
thermo pol buffer | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
DNA pol(VENT) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
dH2O | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 |
TOTAL | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
20,August 2008
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](Luciferase Renilla)-->colony
- [http://partsregistry.org/Part:BBa_I712019 BBa_I712019](Luciferase Fire fly)-->no colony
- [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](LacZ)-->colony
- [http://partsregistry.org/Part:BBa_E2030 BBa_E2030](Venus YFP)-->not so good
- YFP(P-GEX)-->colony
- [http://partsregistry.org/Part:BBa_F2620 BBa_F2620]-->colony
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]-->colony
21,August 2008
- [http://partsregistry.org/Part:BBa_J32007 BBa_J32007](Lux CDABE)-->no colony
- [http://partsregistry.org/Part:BBa_B0034 BBa_B0034](RBS)-->colony
22,August 2008
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](Luciferase)
- [http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]
- [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](LacZ)
- PUC19
- PGEX VENUS YFP(GST fusion)
- PGEX VENUS YFP(GST fusion)
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