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Team:Mississippi State/Maintenance of Plasmids
From 2008.igem.org
Maintenance of Plasmids
- The pPIC6alpha vector contains the blasticidin resistance gene to allow selection of the plasmid using blasticidin. To propogate and maintain the pPIC6alpha plasmid, use the following procedure:
- Resuspend each vector in 20ul sterile water to prepare a 1ug/ul stock solution. Store the stock solution at -20C
- Use the stock solution to transform a recA, endA E.coli strain like TOP10, DH5alpha, JM109, or equivalent.
- Select transformants on Low Salt LB plates containing 100ug/ml blasticidin.
- Prepare a glycerol stock from each transformant containing plasmid for long-term storage.
