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Team:Mississippi State/PCR Gel
From 2008.igem.org
Making PCR Gel
- the Gel is a 1% Agarose gel
- 0.25g/25ml Agarose/TAE
- Weigh 0.25g Agarose into flask(Remember weight).
- Add 25ml TAE.
- Microwave 1min to boil
- wait 7min
- Reweigh
- Add H2O to difference.
- Add 5ul EtBr (Wear Gloves)
- Pour into gel box, inserting templates.
- When gel dries, remove templates and pour TAE until it reaches top of box
- Make sure nodes are on back side
- get ladder (1ul)
- touch to TAE first, then load first well
- get 1ul loading dye, place drop on parafilm
- put 2ul PCR product on drop and mix.
- load 2nd well
- put PCR product on ice(-20C)
- Hook up gel, black-left, red-right; where black is (-) and red is (+).
- set to 80V for 60min.
- Remove gel and take to get picture.
