These tasks are carried out for E7 insert and empty plasmid vector for RBS 0034:

• 4:30 pm: 1st digestion
• 6:30 pm: PCR purification, followed by 2nd digestion
• 11:30 pm: collect digested product and freeze at -20 to prevent reaction.
• Also, at 6:15pm, we plate LacI promoter in Homemade Top 10 and DH5α