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Team:UNIPV-Pavia/Parts
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Assembled Parts Notation
In notebook section we sometimes use abbreviations to name assembled parts. New parts' official name, notebook abbreviations and new parts description are reported in the following table:
| New Part | Notebook Name | Assembled Parts(code) | Assembled Parts(name) | Vector | Description |
|---|---|---|---|---|---|
| - | 01 | J23100-E0240 | Pcon-GFP_protein_generator | J61002 | Test |
| K081005 | 02 | J23100-B0030 | Pcon-RBS | pSB1A2 | Intermediate |
| K081006 | 03 | R0051-B0030 | Plambda-RBS | pSB1A2 | Intermediate |
| S04119 | 04 | B0030-E0040 | RBS-GFP | pSB1A2 | Intermediate |
| K081007 | 05 | B0030-C0051 | RBS-cI | pSB1A2 | Intermediate |
| S04120 | 06 | J23100-E1010 | RBS-RFP | pSB1A2 | Intermediate |
| K081008 | 07 | B0030-C0061 | RBS-luxI | pSB1A2 | Intermediate |
| K081009 | 08 | B0030-C0078 | RBS-lasI | pSB1A2 | Intermediate |
| - | 09 | K081005-C0012 | Pcon-RBS-lacI | pSB1A2 | Intermediate |
| K081010 | 10 | K081005-C0040 | Pcon-RBS-tetR | pSB1A2 | Intermediate |
| - | 11 | K081005-I15010 | Pcon-RBS-cph8 | pSB1A2 | Intermediate |
| K081011 | 12 | K081006-C0062 | Plambda-RBS-luxR | pSB1A2 | Intermediate |
| K081012 | 13 | S04119-B1006 | RBS-GFP-T | pSB1AK3 | GFP protein generator with 39bp artificial terminator |
| K081013 | 14 | K081007-B0030 | RBS-cI-RBS | pSB1A2 | Intermediate |
| K081014 | 15 | S04120-B1006 | RBS-RFP-T | pSB1AK3 | GFP protein generator with 39bp artificial terminator |
| K081015 | 16 | K081008-B1006 | RBS-luxI-T | pSB1AK3 | luxI protein generator RCT |
| K081016 | 17 | K081009-B1006 | RBS-lasI-T | pSB1AK3 | lasI protein generator RCT |
| K081017 | 18 | B0030-I15009 | RBS-pcyA | pSB1A2 | Intermediate |
| - | 19 | (K081005-C0012)-B1006 | Pcon-RBS-lacI-T | pSB1AK3 | lacI protein generator PRCT |
| K081018 | 20 | K081010-B1006 | Pcon-RBS-tetR-T | pSB1AK3 | tetR protein generator PCRT |
| - | 21 | (K081005-I15010)-B0030 | Pcon-RBS-cph8-RBS | ? | Intermediate |
| K081019 | 22 | K081011-B1006 | Plambda-RBS-luxR-T | pSB1AK3 | Plambda regulated luxR protein generator PRCT |
| K081020 | 23 | K081013-C0079 | RBS-cI-RBS-lasR | pSB1A2 | Intermediate |
| K081000 | 24 | K081015-R0062 | RBS-luxI-T-Plux | pSB1A2 | Mux - Channel 0 |
| K081001 | 25 | K081016-R0079 | RBS-lasI-T-Plas | pSB1A2 | Mux - Channel 1 |
| K081021 | 26 | K081017-B1006 | RBS-pcyA-T | pSB1AK3 | pcyA protein generator RCT |
| - | 27 | (K081005-C0012-B1006)-R0010 | Pcon-RBS-lacI-T-Plac | pSB1AK3 | lacI composite part: PRCT-Plac |
| - | 28 | K081018-R0040 | Pcon-RBS-tetR-T-Ptet | pSB1A2 | tetR composite part: PRCT-Ptet |
| - | 29 | (K081005-I15010-B0030)-I15008 | Pcon-RBS-cph8-RBS-ho1 | ? | Intermediate |
| K081022 | 30 | K081019-R0062 | Plambda-RBS-luxR-T-Plux | pSB1A2 | luxR composite part: Plambda regulated PRCT-Plux |
| K081023 | 31 | K081020-B1006 | RBS-cI-RBS-lasR-T | pSB1AK3 | cI-lasR protein generator RCRCT |
| K081024 | 32 | K081021-R0082 | RBS-pcyA-T-Pomp | pSB1A2 | pcyA composite part: RCT-Pomp |
| - | 33 | (K081005-I15010-B0030-I15008)-K081024 | Pcon-RBS-cph8-RBS-ho1-RBS-pcyA-T-Pomp | ? | Light responsive system under constitutive promoter: PRCRCRCT-Pomp |
| K081004 | 34 | K081023-K081022 | RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T-Plux | pSB1AK3 | Demux - Selector |
| K081002 | 35 | K081023-K081019 | RBS-cI-RBS-lasR-T-Plambda-RBS-luxR-T | pSB1AK3 | Mux - Selector |
| K081003 | 36 | K081008-K081001 | RBS-luxI-RBS-lasI-T-Plas | pSB1A2 | Demux - Input |
- 01 part has not been submitted to the registry because it was a test assembly.
- Red-highlighted parts (11, 21, 29, 33) contain I15010 (cph8): according to IGEM DNA Repositories Quality Control (and according to our results) the sequence is inconsistent and so these parts cannot be assembled.
- Green-coloured parts have been succesfully sequenced.
- Blue-coloured parts are long parts and have not been totally sequenced. The sequenced portions were correct.
- Red-coloured parts have been sequenced and a point mutation in C0062 coding sequence (position 349, G->C) was noticed, but these parts seemed to work and so we decided to submit them.
- 24, 25, 34, 35, 36 are parts of our final Mux or Demux systems.
- Sequencing results of all the sequenced parts containing C0061 and C0078 showed that these two coding sequences have an undocumented barcode downstream. This should be documented on C0061 and C0078 Registry pages!
- Parts in gray were programmed, but they have not been assembled and so they haven't been submitted.
