"
Team:University of Ottawa/3 June 2008
From 2008.igem.org
Contents |
Today in the lab
Dan, Matt
- Yesterday's electrophoresis
- The electrophoresis indicated that the miniprep did not go as planned, as a result we will have to do it again. Note: We have to number the colonies that we are using for our miniprep because some may not be expressing the plasmid as well as others.
- We also realized that me might have been using slightly too much DNA in our digestions. We made a calculator to help us determine the right amounts of enzyme, DNA and buffer to use. This should also improve the resolution of our gels.
- New Miniprep
- We begann our new miniprep and will make sure to number the colonies. We prepared the miniprep for each plasmid from two different colonies.
- We left the bacteria to innoculate at 37C overnight at 250 rpm.
