Team:Warsaw/Calendar-Main/14 August 2008
From 2008.igem.org
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Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
Purification of proteins: Z-alpha and Z-omegaPiotrElectrophoresis in polyacrylamide gel (12 %) of sonicate and preliminarily purified protein fusions: Z-alpha and Z-omega (Fig. 1. and Fig. 2.) ![]() 1. Purified Z-omega 2. Supernatant Z-alpha after sonication 3. Elution of Z-alpha using ddH2o 4. Purified Z-alpha 5. Marker ![]() 1. Marker 2. Sonicate Z-omega after induction 3. Pellet Z-omega after induction
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