Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

1. Isolation of plasmids from cultures inoculated on previous day.
2. Control digest of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible).
3. One of positive plasmids transformed into TOP10 and plated on LB+amp, overnight, for further isolation of pET15b+Z+omega vector.

Cloning of protein A DNA to OmpA constructs

Michał K.

1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).
3. Gel electrophoresis - proper clone found.