Rifampicin test #3

Michał L., Ewa, Marcin, Piotr

1. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB.
2. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose.
3. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB.
4. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1 mM IPTG.
5. Cultures were incubated for 5 hours.
6. 1 ml (2x) from p.1, p.2, p.3, p.4 were plated on LB + rifampicin.
7. OD measurement of above cultures.