Team Sorbitol:
The overnight PCR reaction failed.
Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before.
The digestion was verified on a gel and extracted using the Qiagen kit.
This time we obtained enough insert to do a ligation but not enough vector.
To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C
Team Fungus:
Ran 1% agarsoe gel of last nights PCR with plasmid as template. Results were inconclusive.
Ran yet another PCR. Used a touchdown PCR cycle. Running low on cDNA.
Ran another digestion of purfied plasmids. Results inconclusive.
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