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Edinburgh/23 July 2008
From 2008.igem.org
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Week 6
Wednesday 23 July 08
- M43 (BABEL2+glgC-mut1,2) sequence results received. First and second mutation sites successfully removed. (AH)
- Overnight culture of C. fimi did not yield sufficient cells. Made two more cultures in nutrient broth; all cultures incubated at 30°C with shaking. (AM)
- PCR of crtY from P. ananatis cells (P27) and fusion PCR of rbs+crtB and rbs+crtI from ligations L17 and L18 respectively (P28, P29). PCR products were run on Gel 22 - P28 and P29 successful, P27 unsuccessful. (AM, Yan, OG)
- Re-did positive control PCR of rrnB from C. fimi (P30) ~ denaturing at 95°C for 20s, annealing at 55°C for 1 minute, extension at 70°C for 30s. The first two settings were in error: denaturing should have been set to 1 minute and annealing to 10 sec as usual. HOWEVER, annealing for 1 minute appears to have worked, so it might be worth trying this for PCRing the cellulases. (AM)
- P30 (above) and PCR of PzntA were run on Gel 23. Both successful. (AM)
