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Edinburgh/29 June 2008
From 2008.igem.org
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Week 2
Sunday 29 June 08
- Streaks on plate 12 show strong signs of phage infection and are unusable apart from the single white one from plate 5. At least one of the streaks on plate 13 also has a couple of plaques, but plate 11 looks fine. This suggests that phage may have come from the BABEL DNA stock (or the PNK, which seems unlikely). Set up overnight cultures (2.5ml LB in 20ml bijoux) for minipreps. Numbers 1 to 6 are pSB1A2+dxs from plate 11, all white, number 7 is from plate 12 (the white clone from plate 5), and 8 to 12 are from plate 13, white or pale blue. Incubated at 37°C with shaking.
- Also note: a couple more colonies turned up on plates 5 and 6. The one on 6 looks like an escape, but the one on 5 looks OK. Subbed them both to a fresh plate (Plate 14). Previous plates, apart from plates 4 and 8 which had no growth, were transferred to the cold storage room.
