| August
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| M | T | W | T | F | S | S
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| [http://2008.igem.org/Imperial_College/1_August_2008 1]
| [http://2008.igem.org/Imperial_College/2_August_2008 2]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/3_August_2008&action=edit 3]
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| [http://2008.igem.org/Imperial_College/4_August_2008 4]
| [http://2008.igem.org/Imperial_College/5_August_2008 5]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_August_2008&action=edit 6]
| [http://2008.igem.org/Imperial_College/7_August_2008 7]
| [http://2008.igem.org/Imperial_College/8_August_2008 8]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/9_August_2008&action=edit 9]
| [http://2008.igem.org/Imperial_College/10_August_2008 10]
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| [http://2008.igem.org/Imperial_College/11_August_2008 11]
| [http://2008.igem.org/Imperial_College/12_August_2008 12]
| [http://2008.igem.org/Imperial_College/13_August_2008 13]
| [http://2008.igem.org/Imperial_College/14_August_2008 14]
| [http://2008.igem.org/Imperial_College/15_August_2008 15]
| [http://2008.igem.org/Imperial_College/16_August_2008 16]
| [http://2008.igem.org/Imperial_College/17_August_2008 17]
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| [http://2008.igem.org/Imperial_College/18_August_2008 18]
| [http://2008.igem.org/Imperial_College/19_August_2008 19]
| [http://2008.igem.org/Imperial_College/20_August_2008 20]
| [http://2008.igem.org/Imperial_College/21_August_2008 21]
| [http://2008.igem.org/Imperial_College/22_August_2008 22]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/23_August_2008&action=edit 23]
| [http://2008.igem.org/Imperial_College/24_August_2008 24]
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| [http://2008.igem.org/Imperial_College/25_August_2008 25]
| [http://2008.igem.org/Imperial_College/26_August_2008 26]
| [http://2008.igem.org/Imperial_College/27_August_2008 27]
| [http://2008.igem.org/Imperial_College/28_August_2008 28]
| [http://2008.igem.org/Imperial_College/29_August_2008 29]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/30_August_2008&action=edit 30]
| [http://2008.igem.org/Imperial_College/31_August_2008 31]
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| September
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| M | T | W | T | F | S | S
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| [http://2008.igem.org/Imperial_College/1_September_2008 1]
| [http://2008.igem.org/Imperial_College/2_September_2008 2]
| [http://2008.igem.org/Imperial_College/3_September_2008 3]
| [http://2008.igem.org/Imperial_College/4_September_2008 4]
| [http://2008.igem.org/Imperial_College/5_September_2008 5]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_September_2008&action=edit 6]
| [http://2008.igem.org/Imperial_College/7_September_2008 7]
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| [http://2008.igem.org/Imperial_College/8_September_2008 8]
| [http://2008.igem.org/Imperial_College/9_September_2008 9]
| [http://2008.igem.org/Imperial_College/10_September_2008 10]
| [http://2008.igem.org/Imperial_College/11_September_2008 11]
| [http://2008.igem.org/Imperial_College/12_September_2008 12]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/13_September_2008&action=edit 13]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/14_September_2008&action=edit 14]
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| [http://2008.igem.org/Imperial_College/15_September_2008 15]
| [http://2008.igem.org/Imperial_College/16_September_2008 16]
| [http://2008.igem.org/Imperial_College/17_September_2008 17]
| [http://2008.igem.org/Imperial_College/18_September_2008 18]
| [http://2008.igem.org/Imperial_College/19_September_2008 19]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/20_September_2008&action=edit 20]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/21_September_2008&action=edit 21]
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| [http://2008.igem.org/Imperial_College/22_September_2008 22]
| [http://2008.igem.org/Imperial_College/23_September_2008 23]
| [http://2008.igem.org/Imperial_College/24_September_2008 24]
| [http://2008.igem.org/Imperial_College/25_September_2008 25]
| [http://2008.igem.org/Imperial_College/26_September_2008 26]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/27_September_2008&action=edit 27]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/28_September_2008&action=edit 28]
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| [http://2008.igem.org/Imperial_College/29_September_2008 29]
| [http://2008.igem.org/Imperial_College/30_September_2008 30]
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| October
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| M | T | W | T | F | S | S
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| [http://2008.igem.org/Imperial_College/1_October_2008 1]
| [http://2008.igem.org/Imperial_College/2_October_2008 2]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/3_October_2008&action=edit 3]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/4_October_2008&action=edit 4]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/5_October_2008&action=edit 5]
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| [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_October_2008&action=edit 6]
| [http://2008.igem.org/Imperial_College/7_October_2008 7]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/8_October_2008&action=edit 8]
| [http://2008.igem.org/Imperial_College/9_October_2008 9]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/10_October_2008&action=edit 10]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/11_October_2008&action=edit 11]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/12_October_2008&action=edit 12]
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| [http://2008.igem.org/Imperial_College/13_October_2008 13]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/14_October_2008&action=edit 14]
| [http://2008.igem.org/Imperial_College/15_October_2008 15]
| [http://2008.igem.org/Imperial_College/16_October_2008 16]
| [http://2008.igem.org/Imperial_College/17_October_2008 17]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/18_October_2008&action=edit 18]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/19_October_2008&action=edit 19]
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| [http://2008.igem.org/wiki/index.php?title=Imperial_College/20_October_2008&action=edit 20]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/21_October_2008&action=edit 21]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/22_October_2008&action=edit 22]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/23_October_2008&action=edit 23]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/24_October_2008&action=edit 24]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/25_October_2008&action=edit 25]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/26_October_2008&action=edit 26]
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| [http://2008.igem.org/wiki/index.php?title=Imperial_College/27_October_2008&action=edit 27]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/28_October_2008&action=edit 28]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/29_October_2008&action=edit 29]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/30_October_2008&action=edit 30]
| [http://2008.igem.org/wiki/index.php?title=Imperial_College/31_October_2008&action=edit 31]
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9 September 2008
Wet Lab
- Taq PCR trials of genomic sequences set up and results analysed on gel
- Ligations of all available sequences into biobricks set up
A 1% Agarose gel showing the results of various PCR reactions. Each lane is loaded with 5ul of PCR reaction and 1ul of 6x sample buffer. M = Marker
Lane 1 - EpsE 5' IS primers, chromosome template, 52oC for 10 cycles then 64oC for 20 cycles
Lane 2 - EpsE 5' IS primers, chromosome template, 50oC for 10 cycles then 64oC for 20 cycles
Lane 3 - EpsE 5' IS primers, chromosome template, 48oC for 10 cycles then 64oC for 20 cycles
Lane 4 - XylR primers, chromosome template, 50oC for 10 cycles then 60oC for 20 cycles
Lane 5 - XylR primers, chromosome template, 48oC for 10 cycles then 60oC for 20 cycles
Lane 6 - XylR primers, chromosome template, 46oC for 10 cycles then 60oC for 20 cycles
Lane 7 - EpsE 3' IS primers, chromosome template, 54oC for 10 cycles then 64oC for 20 cycles
Lane 8 - EpsE 3' IS primers, chromosome template, 52oC for 10 cycles then 64oC for 20 cycles
Lane 9 - EpsE 3' IS primers, chromosome template, 50oC for 10 cycles then 64oC for 20 cycles
Lane 10 - LacI insert (cut with XbaI and SpeI)
Lane 11 - pSB1AK3 vector (cut with XbaI and SpeI)
Conditions and timings were kept the same as the standard Taq protocol. Pfu PCR reactions using the optimal condiitons can now be set up to produce clones for use in our constructs. From the LacI and pSB1AK3 digests, it is now possible to decide how much of each is required in our ligation reaction.
"
