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Reference
From 2008.igem.org
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1
A sensitive, viable-colony staining method using Nile red
for direct screening of bacteria that accumulate polyhydroxyalkanoic
acids and other lipid storage compounds. Patricia Spiekermann · Bernd H. A. Rehm ·
Rainer Kalscheuer · Dirk Baumeister ·
Alexander Steinbüchel. Arch Microbiol (1999) 171:73–80
This literature explains how pBHR68 works and how PHA granules are detected.
2 Construction and Selection of the Novel Recombinant Escherichia cob Strain for Poly(Hydroxybutyrate) Production.HUIMIN YU,JIN YIN,HONGQI LI,SHENGLI YANG,AND ZHONGYAO. JOURNALOF BIOSCIENCEAND BIOENGWEERING Vol. 89, No. 4, 307-311. 2000
This literature indicates how PHA sysnthesis is realized in Escherichia coli.
3 Spontaneous liberation of intracellular polyhydroxybutyrate granules in Escherichia coli.Il Lae Jung, Ki Heon Phyo, Kug Chan Kim, Hyo Kook Park, In Gyu Kim.Research in Microbiology 156 (2005) 865–873
This literature introduced a method to release PHB granules from Escherichia coli.
4 Simultaneous Expression of Vitreoscilla Globin Gene and Lytic Genes of Phage lambda Novel RecombinantEscherichia Coli Used for Production of PHB.YU Huimin,SHI Yue,YIN Jin,and SHEN Zhongyao.ChineseJ.ofChem.Eug，(4)407一411(2001)
This literature introduced phage lambda lytic gene into Escherichia coli strains.
5 Autoregulator Protein PhaR for Biosynthesis of Polyhydroxybutyrate [P(3HB)] Possibly Has Two Separate Domains That Bind to the Target DNA and P(3HB): Functional Mapping of Amino Acid Residues Responsible for DNA Binding.Miwa Yamada,Koichi Yamashita,Akiko Wakuda,Kazuyoshi Ichimura,Akira Maehara,Michihisa Maeda,and Seiichi Taguchi.JOURNAL OF BACTERIOLOGY, Feb. 2007, p. 1118–1127
This literature illustrates how PhaR works in the PHB synthesis regulation.
6 Influence of homologous phasins (PhaP) on PHA accumulation and regulation of their expression by the transcriptional repressor PhaR in Ralstonia eutropha H16.Markus Po¨tter, Helena Muller and Alexander Steinbuchel.Microbiology (2005), 151, 825–833.
This literature illustrates in the interaction of PhaP and PhaR.
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