Team:Chiba/Calendar-Home/23 August 2008

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22 August 2008 <|> 24 August 2008

Contents

Meeting with UmeG

the ppt for our weekly meeting(japanese)

  1. Team:Input
  2. Team:Communication
  3. team:Output


Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)


--->Digestion test
  1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)


Sample No. 1- 671 - 71 - 7
DNA(μL) 3133
EcoRI(μL) --0.10.1
PstI(μL) --0.10.1
Buffer EcoRI(μL) --0.90.8
BSA(μL) --11
dH2O(μL) --55
TOTAL(μL) 311010


--->Gel Check
Chiba-0823-1.JPG
Sample No. 1~67
Sample DNA 31
Loading Dye(μL) 11
dH2O(μL) 24
TOTAL(μL) 66
From left; Sanple No. 1~7
  1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)



Chiba-0823-2.JPG
Sample No. 8~21
Sample DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12
From left;
(Single Digestion) Sanple No. 8~14
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
(Double Digestion) Sample No. 15~21
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK?



Digestion test
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007:old-sample)
Sample No. 123
Sample DNA(μL) 333
EcoRI(μL) -0.10.1
SpeI(μL) --0.1
EcoRI Buffer(μL) -0.90.8
BSA(μL) -11
dH2O(μL) -55
TOTAL(μL) 31010


--->Gel Check

Chiba-0823-3.JPG
Sample No. 123
Sample DNA 31010
Loading Dye(μL) 122
dH2O(μL) 2--
TOTAL(μL) 61212
From left;
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Single Digestion -> OK
  3. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Double Digestion -> Bad

Team:Output

no work

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