Team:Chiba/Calendar-Home/24 August 2008

From 2008.igem.org

>Home | Notebook

23 August 2008 <|> 25 August 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

(22/8)--->Mini prep

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)

--->Digestion test

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006)

Sample No.	5	6	7	8
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)(μL)	1	-	-	-
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006)(μL)	-	1	-	-
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(μL)	-	-	1	-
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007)(μL)	-	-	-	1
EcoRI(μL)	0.1	0.1	0.1	0.1
Buffer 4(μL)	0.9	0.9	0.9	0.9
dH₂O(μL)	8	8	8	8
TOTAL(μL)	10	10	10	10

--->Gel Check

Sample No.	1~8
Sample DNA(μL)	1
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

From left;

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> ?
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> OK
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> None?

Sample No.	5~8
Sample DNA(μL)	9
Loading Dye(μL)	2
dH₂O(μL)	1
TOTAL(μL)	12

From the left; （single digestuion）

5, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK

6, [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2006) -> OK

7, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2007) -> 3400bp:Bad, 2200bp:OK

8, [http://partsregistry.org/Part:BBa_J13002 BBa_J13002](2006) -> OK

Team:Output

[http://partsregistry.org/Part:BBa_J63001 BBa_J63001]-->colony

[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)
Menbren Venus YFP(3)

Sample No.	1	2	3
DNA template(μL)	3	3	3
Buffer3(μL)	1	1	1
dH₂O(μL)	6	6	6
EcoRI(μL)	0.5	0.5	0.5
TOTAL(μL)	10	10	10

-->37°C 30min

Sample No.	1(afetr digestion)	2(after digestion)	3(afetr digestion)
DNA tamplate(μL)	10	10	10
loading Dye(μL)	2	2	2
TOTAL(μL)	12	12	12

Sample No.	1	2	3
DNA tamplate(μL)	2	2	2
loading Dye(μL)	1	1	1
dH₂O(μL)	3	3	3
TOTAL(μL)	6	6	6

-->135V 30min

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/24_August_2008"